mouse ADCC Bioassay Effector Cell
CBP74076
| I. Background | |
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Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism involving an effector cell lysing a target cell on which antibodies have bound to specific antigens on the target cell membrane. The typical ADCC involves activation of natural killer (NK) cells by antibodies. NK cells express Fc receptors, mostly CD16 or FcγRIIIa (CD16a), on its cell surface. These Fc receptors recognize and bind to the Fc portion of an antibody, such as IgG, which has bound to the surface of a pathogen-infected target cell. Once the Fc receptor binds to the Fc region of IgG, the Natural Killer cell releases cytokines such as IFN-γ and cytotoxic molecules that attack the pathogen-infected target cell. |
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| II. Description | |
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Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of Mouse FcγRIV.
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| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | NFAT |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800ug/ml hygromycin+1ug/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute T cell leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 2. Dose response of mouse Anti-CD20 lgG2a in Mouse ADCC Bioassay Effector Cell (C3) . Raji cells as target cells.
