CCR4/β-Arrestin/CHO

CBP71349

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I. Background
C-C chemokine receptor type 4 is a protein that in humans is encoded by the CCR4 gene.The protein encoded by this gene belongs to the G protein-coupled receptor family. It is a receptor for the following CC chemokines:CCL2 (MCP-1) CCL4 (MIP-1) CCL5 (RANTES) CCL17 (TARC) CCL22 (Macrophage-derived chemokine). Chemokines are a group of small structurally related proteins that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

II. Introduction
Host Cell:	CHO
Stability:	20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	F12K+10%FBS+5ug/ml puromycin+5ug/ml blasticidin
Mycoplasma Status:	Negative
Storage:	Liquid nitrogen immediately upon delivery
Application(s):	Functional assay for CCR4 receptor
Transducer：	Gi

Ⅲ. Description of Host Cell Line
Organism:	Hamster
Tissue:	Ovary
Morphology:	Epithelial
Growth Properties:	Adherent

Ⅳ. Representative Data

Figure 2. Dose response of Human Recombinant CCL22 in CHO-K1 CCR4 β-Arrestin Cell Line (C12).
Figure 3. Inhibition of CCL22-induced beta-Arrestin Recruitment in CCR4 beta-Arrestin CHO-K1 Cells..