EFM-19（人乳腺癌細(xì)胞）
CBP60363
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| I. General information | |
| Synonyms: | EFM-19 |
| Background: | established from the pleural effusion of a 50-year-old Caucasian woman with breast carcinoma (ductal type, histological grading II) in 1979 |
| Species: | human (Homo sapiens) |
| Tissue: | breast |
| Disease: | breast carcinoma |
| Gender: | N/A |
| Morphology: | epitheloid, growing in clusters at first, then as monolayer; |
| Growth Mode: | N/A |
| Doubling Time: | 50-60 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS EFM-19完全培養(yǎng)基，# CBP60363M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin - |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, MDH |
| Cytogenetics: | human highly rearranged hypotriploid karyotype with 5.8% polyploidy - 62(58-65)<3n>X, der(X), apparent ch 17 nullisomy and preponderance of marker chromosomes, e.g. add(1)(p11), idel(1)(q11q?22), i(1p), i(2p), add(4)(p11), add(4)(p11), etc. - resembes original description |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
