SV40LT-SMC Clone HEP-SA （大鼠平滑肌細胞）
CBP60611
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| I. General information | |
| Synonyms: | SV40LT-SMC Clone HEP-SA |
| Background: | This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. |
| Species: | Rattus norvegicus, rat |
| Tissue: | aorta; smooth muscle |
| Gender: | 3 months, rat |
| Morphology: | fibroblast |
| Growth Mode: | adherent |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+10%FBS+200ug/ml G418 SV40LT-SMC Clone HEP-SA 完全培養(yǎng)基，# CBP60611M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | alpha actin; large T antigen |
| Cellular Products: | alpha actin; large T antigen |
| Comments: | This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance). Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin. Large T antigen is detectable in the nuclei. For more information, please contact Cobioer (4008-750-250). |
