LS513（人盲腸癌細(xì)胞）
CBP61154
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| I. General information | |
| Synonyms: | LS513 |
| Background: | LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve. |
| Species: | Homo sapiens, human |
| Tissue: | cecum |
| Disease: | Dukes' type C,colorectal carcinoma |
| Gender: | male， 63 years， Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 25 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,10 D16S539: 12,13 D5S818: 9,11 D7S820: 8,11 THO1: 8 TPOX: 8 vWA: 16,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS LS513完全培養(yǎng)基，# CBP61154M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive |
| Receptor Expression: | N/A |
| Karyotype | N/A |
| Virus Susceptibility | Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1 |
| Oncogene: | p53 + (wild type) |
| Genes Expressed: | transforming growth factor beta 1 (TGF beta-1, 83 pg per 106 cells per 24 hours) |
| Cellular Products: | transforming growth factor beta 1 (TGF beta-1, 83 pg per 10 exp6 cells per 24 hours) |
| Tumor Formation: | Yes, forms tumors in nude mice |
| Comments: | LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve. Approximately 50% of LS513 cells express surface carcinoembryonic antigen (CEA). LS513 cells express the major histocompatibility (MHC) class I antigens HLA and beta 2 microglobulin. MHC class II antigens (HLA-DR, DQ, and DP were not detected). TGF beta-1 is inhibitory for proliferation of LS513 cells, whereas TGF beta-2 has no effect on the growth of these cells. LS513 cells are 100-fold less sensitive to TGF beta-1 than the LS1034 (ATCC CRL-2158) cell line. LS513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice. Colony forming efficiency was 30% in methylcellulose. For more information, please contact Cobioer (4008-750-250). |
