Super Tube（狗腎上皮細(xì)胞）
CBP61165
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產(chǎn)品描述
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| I. General information | |
| Synonyms: | Super Tube |
| Background: | Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. |
| Species: | Canis familiaris, dog |
| Tissue: | N/A |
| Disease: | N/A |
| Gender: | female, adult |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | N/A |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM:Ham's F12(1:1)+0.5 mM sodium pyruvate+0.05 mM non-essential amino acids+10%FBS Super Tube完全培養(yǎng)基，# CBP61165M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | N/A |
| Receptor Expression: | N/A |
| Karyotype | N/A |
| Virus Susceptibility | N/A |
| Oncogene: | N/A |
| Genes Expressed: | N/A |
| Cellular Products: | N/A |
| Tumor Formation: | N/A |
| Comments: | Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate). For tubule formation, the cells should be fed twice a day by a complete medium change. Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK. For more information, please contact Cobioer (4008-750-250). |
