SNU-182（人肝癌細(xì)胞）
CBP60211
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| I. General information | |
| Synonyms: | SNU-182 |
| Background: | SNU-182 was derived in 1989 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy. |
| Species: | Homo sapiens, human |
| Tissue: | liver |
| Disease: | grade III/IV, hepatocellular carcinoma |
| Gender: | male, 24 years, Asian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 46 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,14 D16S539: 11,13 D5S818: 11,12 D7S820: 11 THO1: 9 TPOX: 8,11 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS SNU-182完全培養(yǎng)基，# CBPCBP60211M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | Blood Type O; Rh + |
| Comments: | Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. Grossly, the original tumor was single nodular. Histologically, it was predominantly trabecular and minor acinar type. The cultured cells contain a single nucleus. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed. For more information, please contact Cobioer (4008-750-250). |
