hFGFR2b Effector Reporter Cell
CBP74196
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| I. Background | |
| FGFR2b has three structured domains: extracellular, transmembrane, and cytoplasmic tyrosine kinase. The extracellular domain is an immunoglobulin-like structure with three immunoglobulin (Ig) homology loops (I, II, III), which determines the ligand-binding specificity. The transmembrane domain of FGFR2b anchors the receptor with the cell membrane. The intracellular domain of receptors displays tyrosine kinase activity, phosphorylating the downstream targets and initiating intracellular signaling | |
| II. Introduction | |
| Expressed gene: | FGFR2b |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+100ug/ml hygromycin+2ug/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
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Figure 1. Dose Response of Recombinant Human FGF-7&FGF-10 in hFGFR2b Effector Reporter Cells (C1C4). |
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Figure 2. Inhibition of hFGF-7-Induced Reporter Activity by FGFR2b Blocking Ab in hFGFR2b Effector Reporter Cells (C1C4). |
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Figure 3. Inhibition of hFGF-10-Induced Reporter Activity by FGFR2b Blocking Ab in hFGFR2b Effector Reporter Cells (C1C4). |
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