OCI-AML2（人急性髓系白血病）
CBP60527
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| I. General information | |
| Synonyms: | OCI-AML2 |
| Background: | established from the peripheral blood of a 65-year-old man with acute myeloid leukemia (AML FAB M4) in 1986 at diagnosis; cells were described in the literature to express the retinoic acid receptor gene; cell line carries the DNMT3A R635W mutation |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | acute myeloid leukemia |
| Gender: | N/A |
| Morphology: | single, round to oval cells in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | about 30-50 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
80% MEM α (GIBCO 12561-056 ) + 20% FBS OCI-AML2完全培養(yǎng)基，# CBP60527M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD68 +, HLA-DR (+); image |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of MDH, NP |
| Cytogenetics: | human hyperdiploid karyotype with 3.8% polyploidy - 48(43-49)<2n>XY, +6, +8, der(1)inv(1)(p36q31)t(1;6) (q13;p12), der(2)t(2;17)(p23;q24.1)del(2)(q14.2q36), der(3)t(1;3)(p36;p25), ins(3;2)(q21;q14.2q36), t(5;8)(q11.2;q24), der(6)t(1;6)(q31;p12)t(3;6) (q26;q24), inv(12)(p13.3q13.2), t(13;14)(q32/33;q24.2), der(17)t(2;17)(p23;q24.1) - sideline with +der(5) - carries apparent variant translocations involving several ANLL breakpoints: 1p36, 3q21, 3q26 (megakaryocytic abnormalities), 12p13, 17q24 (FAB-M4) |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
