OCI-AML5（人急性髓細(xì)胞白血?。?/h1>
CBP60528
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| I. General information | |
| Synonyms: | OCI-AML5 |
| Background: | established from the peripheral blood of a 77-year-old man with acute myeloid leukemia (AML M4) in relapse in 1990; the cells are described in the literature to respond to G-CSF, GM-CSF, IL-3, and FLT3 ligand; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to rapid outgrowth of independent cells |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | acute myeloid leukemia |
| Gender: | N/A |
| Morphology: | round cells growing singly or in clumps in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | about 30-50 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
80% alpha-MEM(GIBCO 32561037 )+ 20% FBS + 10 ng/ml GM-CSF OCI-AML5完全培養(yǎng)基，# CBP60528M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR +; |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, LDH, NP |
| Cytogenetics: | human hyperdiploid karyotype - 48(44-48)<2n>XY, +1, +8, der(1)t(1;19)(p13;p13) |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
