LS174T（人結(jié)腸癌腫瘤細(xì)胞）
CBP60033
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| I. General information | |
| Synonyms: | LS174T |
| Background: | The cell line was established from a Duke's type B adenocarcinoma of the colon. The tissue was minced and cultured without transfer for 10 months. |
| Species: | Homo sapiens, human |
| Tissue: | colon |
| Disease: | Dukes' type B,colorectal adenocarcinoma |
| Gender: | female, 58 years,Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 10, 13, 14 D13S317: 10 D16S539: 11, 13 D5S818: 11, 15, 16 D7S820: 10.3, 11 THO1: 6, 7 TPOX: 8, 9 vWA: 15, 17, 18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | serologically defined colon cancer antigen 3; Homo sapiens, expressed HLA A2, B13, B50; Blood type O |
| Oncogene: | myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src - |
| Genes Expressed: | carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), mucin.The production of CEA in the ATCC seed stock was 1944 ng per 10(6) cells in 10 days. |
| Cellular Products: | carcinoembryonic antigen (CEA) interleukin 10 (IL-10) interleukin 6 (IL-6) mucin |
| Tumor Formation: | Yes, in nude mice. (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments: | LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA).Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. Ref They are negative for p53 antigen expression, but positive for mRNA expression.LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes. Ref.K-ras and sis oncogene expression were not detected. For more information, please contact Cobioer (4008-750-250). |
